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. 1987 Oct;55(10):2416–2419. doi: 10.1128/iai.55.10.2416-2419.1987

Monoclonal antibody to human renal glomeruli cross-reacts with streptococcal M protein.

P Goroncy-Bermes 1, J B Dale 1, E H Beachey 1, W Opferkuch 1
PMCID: PMC260723  PMID: 2443450

Abstract

Two murine monoclonal antibodies (immunoglobulin M) evoked against human kidney tissue were examined for cross-reactivity with group A streptococci. A glomerulus-specific antibody, PMII.40.H.2, cross-reacted in an enzyme-linked immunosorbent assay with purified pepsin-extracted M proteins from type 6 and 12 streptococci, but not type 1, 3, 5, 19, and 24 M proteins. The cross-reactive monoclonal antibody also opsonized type 6 and 12 streptococci, indicating that it was directed against protective M protein epitopes that were exposed on the surfaces of these organisms. A control antibody, which was tubule specific, did not cross-react with any of the purified M proteins, nor did it opsonize any of the serotypes of streptococci tested. Western immunoblot experiments identified the glomerular cross-reactive antigen as a 43-kilodalton protein. The results demonstrate that M protein of group A streptococci and glomerular antigens of the human kidney possess cross-reactive determinants.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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