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. 1972 Jul;69(7):1905–1910. doi: 10.1073/pnas.69.7.1905

Deletion Mutants of Bacteriophage ϕX174

Anthony J Zuccarelli 1, Robert M Benbow 1, Robert L Sinsheimer 1
PMCID: PMC426829  PMID: 4558663

Abstract

Mutants of bacteriophage ϕX174 have been isolated that are less dense than wild-type ϕX particles in CsCl. When mutant viral (+) strand DNA and wild-type complementary (-) strand DNA are hybridized, the resulting duplex molecules have single-stranded loops characteristic of wild-type-deletion heteroduplexes. The mutant bacteriophages fail to complement ϕX amber mutants in cistron E. We conclude that the mutant viruses have deleted approximately 7% of the ϕX genome in the region of cistron E.

Keywords: electron microscopy, DNA heteroduplex, density gradient centrifugation, polyacrylamide gel electrophoresis

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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